Fig. 6

Legend. Anti-IL-18 mAb treatment during DNA vaccination decreases therapeutic effects against TC-1 tumor in C57BL/6 mice. One day prior to all of the vaccinations, Tc-1-treated mice (n = 3) were injected intra-peritoneally (i.p) with 100 mg of anti-mouse IL-18 or isotype control mAb. One week following the final blockade and vaccine administration, the tumor volume was monitored up to 6 weeks after the tumor challenge. Line and scatter plot graphs depicting the tumor volume (in mm3) are presented. The data presented are a representation of three independent experiments. Bar graph (Mean ± SD) showing average tumor volume on the day of 28. *** Indicates statistically significant difference (P < 0.001) between E7 DNA vaccine plus α-GalCer and MPL combination mice in the presence of anti-IL-18 mAbs over E7 DNA vaccine plus α-GalCer and MPL combination in the presence of isotype control