Fig. 7

Caspase activity assay in MDA-MB-231 cells. Treated (0–60 μM) and untreated cells for 24 h were tested for fluoremetric assay to determine the levels of activated caspase-9 and caspase-3. a caspase-9 and b caspase-3 activities were enhanced by andrographolide dose dependently. c Cells were treated with andrographolide, or andrographolide + caspase inhibitors (50 μM) at the indicated dose and time period. All of the caspase inhibitors significantly inhibited andrographolide-induced apoptosis. All data represent mean ± S.D. from 3 independent experiments (*P < 0.05, **P < 0.01 and ***P < 0.001)