Fig. 4

HE-Et and HE-My alleviate amyloid plaque-associated glial activation in the area including hippocampal and cortex of APP/PS1 mice. Five month-old APP/PS1 mice were orally administered with vehicle (Veh, n = 6), HE-Et (n = 5) and HE-My (n = 6) for 30 days. Amyloid plaques were detected by immunohistochemical staining with AB-10 antibody (blue). Microglia and reactive astrocytes were detected by immunohistochemical staining with Iba-1 antibody (red) and GFAP antibody (green), respectively. a. The representative immunostaining images of hippocampus and partial cortex. Sale bar: 500 μm. b. The enlarged views of a typical cluster which is indicated in panel a (arrow). Sale bar: 50 μm. c. Both the number of microglial and astroglial cluster in semi-cerebral sphere calculated by image analysis software. d. The immunoblotting of Iba-1 and GFAP. Representative immunoblots of the Iba-1, GFAP and β-actin were showed (left panel). The relative levels of Iba-1 and GFAP were expressed as percentage of control (Veh) (right panel). The results are the mean ± S.D. Significant differences between treated and control (Veh) groups are indicated by *, p < 0.05; **, p < 0.01; ***, p < 0.001