Fig. 6

HE-My and HE-Et promotes hippocampal neurogenesis in APP/PS1 mice. Five months old wild type (WT) or APP/PS1 mice were orally administered with vehicle (Veh, n = 6), HE-Et (n = 6) and HE-My (n = 6) for 30 days. Hippocampal neurogenesis was detected by immunohistochemical staining with doublecortin (DCX) antibody (red) and BrdU antibody (green). a. The representative immunostaining images of dentate gyrus in wild type (WT, n =7) mice and APP/PS1 mice treated with vehicle (Veh), HE-Et and HE-My. Arrow indicates DCX-labeled newly born neuron; arrow head indicates proliferating type 2 neuroprogenitor; double arrow head indicates the newly born neuron immediately after proliferation; hollow arrow indicates proliferating cells other than neuroprogenitor. Scale bar: 100 μm. ML, molecular layer; UGL, upper blade granular cell layer; LGL, SGZ, subgranular zone; lower blade granular cell layer. b. The number of BrdU positive cells, doublecortin positive cells and the cells with double labeling (cell number/mm SGZ). The results are the mean ± SD. Significant differences between wild type (WT) and APP/PS1 groups are indicated by *, p < 0.05. Significant differences between treated and control (Veh) groups are indicated by #, p < 0.05; ##, p < 0.01