Fig. 2

Assessment of autophagy in different stages of THP-M-derived foam cell formation. a Western blot analysis of p62 and LC3II/LC3I after THP-M incubation with oxLDL for 0 h, 6 h, 24 h, 48 h, and 72 h. The ratios of the mean grayscale of LC3I, LC3II, and P62 to GAPDH among groups are shown in (b); (c) Ad-LC3-GFP-mRFP infection measure autophagic flux intensity quantitative analysis; (d) the amounts of autolysosomes (red) and autophagosomes (yellow). The data are presented as mean ± SEM. ^*,§ P < 0.05 vs control group