Fig. 5

Thsd7a knockdown resulted in aberrant Notch1b expression pattern. Whole-mount ISH revealed the spatiotemporal expression patterns of notch1b at 48 hpf with either Thsd7a MO1 (a’-c’) or 5msMO1 (a-c). In the control group, notch1b transcripts were detected in the hind brain with a grid-like pattern (a). In comparison, Thsd7a morphants lost the grid-like expression pattern of notch1b (a’), which were indicated by the white arrows. Reduced expression of notch1b in the spinal cord (b’) was observed. In Thsd7a morphants, notch1b ectopic expression was observed throughout the endocardium in the heart tube (c’) indicated by white arrow head