Fig. 5

Biotransformation capacity and iNOS expression in the livers. C57BL/6 N mice were treated either with LPS (5 mg/kg body weight), AMD3100 (5 mg/kg body weight), with both substances or with the solvent PBS (control). 24 h thereafter, the mice were sacrificed and the livers were collected for biochemical and histological analysis. Ethoxycoumarin-O-deethylation [ECOD] (a), methoxyresorufin-O-demethylation [MROD] (b) and p-nitrophenol-hydroxylation [PNPH] (c) activities in 9000 g supernatants are shown exemplarily. Data are given as mean ± standard error of the mean (SEM); n = 7 for each group. Statistical significant differences between the different treatment groups were determined by using the one-way analysis of variance (ANOVA) and the Tukey post hoc test and are indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. control animals; ⁺, p < 0.05; ⁺⁺, p < 0.01; ⁺⁺⁺, p < 0.001 vs. LPS treatment. d: CYP2B and CYP2E1 expression in liver tissue as determined by immunoblotting. Representative examples of n = 4 independent immunoblots are depicted. e and g: CYP 2B isoforms expression in liver tissue as determined by immunohistochemistry (red-brown color, counterstaining with hematoxylin). Representative photomicrographs from one of seven different liver tissue samples are shown (original magnification: 200×). h and i: iNOS expression in liver tissue as determined by immunohistochemistry (red-brown color, counterstaining with hematoxylin). Representative photomicrographs from one of seven different liver tissue samples are shown (magnification: 630×, arrowheads: iNOS expressing infiltrating neutrophil granulocytes)