Fig. 7

Spleen weights and CD3 and cleaved caspase-3 expression in the spleen. C57BL/6 N mice were treated either with LPS (5 mg/kg body weight), AMD3100 (5 mg/kg body weight), with both substances or with the solvent PBS (control). 24 h thereafter, the mice were sacrificed and the spleens were collected for further analysis. a: Spleen weights. Data are given as mean ± standard error of the mean (SEM); n = 7 for each group. Statistical significant differences between the different treatment groups were determined by using the one-way analysis of variance (ANOVA) and the Tukey post hoc test and are indicated as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ⁺, p < 0.05; ⁺⁺, p < 0.01; ⁺⁺⁺, p < 0.001 vs. LPS. b: CD3 and cleaved caspase-3 expression in the spleen as determined by immunoblotting. Representative immunoblots of n = 4 independent analyses are shown. c-f: cleaved caspase-3 expression in the spleen as determined by immunohistochemistry (red-brown color, counterstaining with hematoxylin). Representative photomicrographs from one of seven different tissue samples each are shown (original magnification: 200×)