Fig. 5
From: Therapeutic effect of berberine on TDP-43-related pathogenesis in FTLD and ALS
Inhibition of autophagy reverses the effect of berberine on TDP-43 aggregates elimination. a and b After 24 h transfection, N2a cells transfected with GFP-TDP-25 were treated with 20 μg/ml berberine and autophagy inhibitor 10 μM 3-MA for another 24 h, and stained with anti-GFP (green) and anti-LC3-II (red). c and d The soluble/insoluble TDP-43 were extracted by RIPA/urea fractionation and analyzed by Western blotting with anti-GFP and anti-LC3 antibodies