Fig. 2
From: Loss of porin function in dopaminergic neurons of Drosophila is suppressed by Buffy
Loss of porin activity decreases survival and impairs climbing ability. a The inhibition of porin in neurons using the Ddc-Gal4 transgene results in decreased median lifespan of 48 and 52 days when compared to 68 days for control flies that expresses UAS-lacZ. The genotypes are Ddc-Gal4/ UAS-lacZ, Ddc-Gal4/ UAS-porin-RNAi (1) and Ddc-Gal4/ UAS-porin-RNAi (2). Longevity is shown as percent survival (P < 0.05, determined by the log-rank (Mantel-Cox) test and n ≥ 200). b The inhibition of porin in the Ddc-Gal4-expressing neurons resulted in a significant decrease in climbing ability as determined by nonlinear fitting of the climbing curves and comparing 95% CI. The genotypes are Ddc-Gal4/ UAS-lacZ, Ddc-Gal4/ UAS-porin-RNAi (1) and Ddc-Gal4/ UAS-porin-RNAi (2). Error bars indicate SEM and n = 50. c The co-expression of Buffy with porin-RNAi result in the rescue of the observed phenotype of decreased survival, with a median survival of 70 and 69 days when compared to 72 days for controls. Genotypes are Ddc-Gal4 UAS-Buffy/ UAS-lacZ, Ddc-Gal4 UAS-Buffy/ UAS-porin-RNAi (1) and Ddc-Gal4 UAS-Buffy/ UAS-porin-RNAi (2). Longevity is shown as percent survival (P < 0.05, determined by log-rank (Mantel-Cox) test with n ≤ 200). d The inhibition of porin along with the overexpression of Buffy in the DA neurons results in the suppression of the age-dependent loss in climbing ability. The genotypes are Ddc-Gal4 UAS-Buffy/ UAS-lacZ, Ddc-Gal4 UAS-Buffy/ UAS-porin-RNAi (1) and Ddc-Gal4 UAS-Buffy/ UAS-porin-RNAi (2). Analysis was done by nonlinear fitting of the climbing curves and significance was determined by comparing the 95% CI. Error bars indicate SEM and n = 50