Fig. 6

OxLDL-induced JAB1 and NF-κB signaling in human MФ. PMA-differentiated human U937 MΦ were treated (4 h) with or without 50 μg/ml oxLDL or 0.1 μg/ml LPS. The protein expression level of (a) Iκ-Bα and (b) NF-κB p65 were determined using western blot analysis and quantified by ImageJ. Expression was normalized against α-tubulin. Bars represent mean ± SEM of 6 (a) or 3 (b) independent experiments. *p < 0.005 significance vs. control. c Cytosolic protein [C] and nuclear protein [N] were extracted and western blot analysis was performed for the NF-κB p65 subunit and quantified by ImageJ (Bars represent mean + SEM of 5 independent experiments). d Immunoprecipitation of JAB1 was carried out with anti-JAB1 [2A10.8] (GenTex) antibodies followed by immunoblot detection for JAB1/COPS5, IKKß, Iκ-Bα and NF-κB in PreClear cell lysates and immunoprecipitates (IP). The eluate from Control Agarose Resin served as negative control. The depicted blot is representative for 3 independent experiments