Marrow adipocytes inhibit the differentiation of mesenchymal stem cells into osteoblasts via suppressing BMP-signaling

Table 1 Down-regulation of osteogenic genes expression in mBMSCs treated with CM-Adipo as compared to CM-Control

Gene name	Gene symbol	Fold change
Ossification and matrix molecules
Alkaline phosphatase, liver/bone/kidney	Alpl	−6.8
Bone gamma carboxyglutamate protein	Bglap	−3.2
Biglycan	Bgn	−4.5
Cadherin 11	Cdh11	−5.0
Chordin	Chrd	−4.3
Collagen type I alpha 1	Col1a1	−4.2
Collagen type I alpha 2	Col1a2	−2.4
Collagen type V alpha 1	Col5a1	−5.5
Secreted phosphoprotein 1 (Osteopontin)	Spp1	−5.8
BMP signaling pathway
Bone morphogenetic protein 2	Bmp2	−6.8
Bone morphogenetic protein 7	Bmp7	−3.1
Bone morphogenetic protein receptor. type 1A	Bmpr1a	−4.5
MAD homolog 5 (Drosophila)	Smad5	−3.2
Cell Adhesion Molecules
Fibronectin 1	Fn1	−6.2
Integrin beta 1 (fibronectin receptor beta)	Itgb1	−2.1
Integrin alpha 2	Itga2	−3.7
Integrin alpha 2b	Itga2b	−3.1
Osteogenic growth factors
Fibroblast growth factor receptor 2	Fgfr2	−2.6
Insulin-like growth factor 1	Igf1	−9.2
Insulin-like growth factor I receptor	Igf1r	−4.1
Platelet derived growth factor. alpha	Pdgfa	−2.9
Osteogenic transcription factors
Distal-less homeobox 5	Dlx5	−7.7
Runt related transcription factor 2	Runx2	−6.2
Sp7 transcription factor 7	Sp7	−4.3

Cells were cultured in either 100% CM-Adipo or CM-Control and induced to differentiate into osteoblasts as described in the Methods. Mouse osteogenesis RT² Profiler™ PCR array with 84 osteoblast genes was performed for each cDNA sample using the SYBR® Green quantitative PCR method. Each target gene was normalized to reference genes and the differentially down-regulated genes by BMSCs in CM-Adipo were represented as fold change in the Table. Values are mean of three independent experiments

ISSN: 1423-0127