Fig. 6
From: Adducin family proteins possess different nuclear export potentials
CRM1 interacts with all ADD proteins, whereas exportin5 preferentially interacts with ADD2. a HA-ADD1, HA-ADD2, and HA-ADD3 were transiently expressed in HEK293 cells and their expression was analyzed by immunoblotting (IB) with anti-HA antibody. CRM1 was immunoprecipitated (IP) with anti-CRM1 antibody or pre-immune serum (IgG) as the control. The immunocomplexes were analyzed by immunoblotting with anti-HA and anti-CRM1 antibodies. b HA-ADD1, HA-ADD2, and HA-ADD3 were transiently expressed in HEK293 cells and their expression was analyzed by immunoblotting (IB) with anti-HA antibody. Exportin5 was immunoprecipitated (IP) with anti-exportin5 antibody or pre-immune serum as the control. The immunocomplexes were analyzed by immunoblotting with anti-HA and anti-exportin5 antibodies. WCL, whole cell lysates