Fig. 4

PGIS-overexpressing OEC cocultures enhanced neuronal connection and reduced microglial number in spinal cord neuron-glial cultures. (a & d): neuron-glial cultures only, (b & e): neuron-glial cultures cocultured with Con-OEC, (c & f): neuron-glial cultures cocultured with PGIS-OEC, (g & h): quantitative bar charts for tubulin immunoreactivity and ED1 (+) cell numbers, respectively. A-C are tubulin immunoreactive cells and D-F are ED1 (+) microglial cells. Bar = 100 μm. *,** P < 0.01,0.05, compared to neuron-glial cultures, by one-way analysis of variance + Bonferroni post test. Data were expressed as mean ± SEM, represent the results of two independent experiments performed in duplicate-triplicate