Fig. 3

Effect of miR-141 on OS cell proliferation, invasion and autophagy. a The expressions of miR-141 in OS cell lines (U2OS and MG63) and osteoblastic cell line HFOB1.19 were analyzed by qRT-PCR. GAPDH was used as the endogenous control. Cell proliferation in U2OS cells (b) transfected with miR-141 or miR-control and MG63 cells (c) transfected with anti-miR-141 or anti-miR-control at 24 h, 48 h, 72 h and 92 h was examined by MTT assay. The number of invasive cells in U2OS cells (d) transfected with miR-141 or miR-control and MG63 cells (e) transfected with anti-miR-141 or anti-miR-control were determined by Transwell invasion assay. The levels of Atg5, LC3-I, LC3-II and p62 in U2OS cells (f) transfected with miR-141 or miR-control and MG63 cells (g) transfected with anti-miR-141 or anti-miR-control were detected by western blot. β-actin was used as the internal control. *P < 0.05 vs. control group