Fig. 7
The A2AR and baicalin attenuated PI3K phosphorylation and AKT phosphorylation in the hypoxia-induced PAH mouse model. Phospho-PI3K levels and PI3K protein expression levels in lung tissues were examined by western blot. GAPDH served as an internal control (a, b; n = 3). Phospho-AKT levels and AKT protein expression levels in lung tissues were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Data are presented as the mean ± SD. #Value significantly greater than the corresponding value in saline-treated normoxic mice (# P < 0.05, ## P < 0.01). *Value significantly less than the corresponding value in hypoxic mice (*P < 0.05, **P < 0.01). §Value significantly less than the corresponding value in baicalin-treated mice (§§ P < 0.01). +Value significantly greater than the corresponding value in WT saline-treated mice, WT hypoxic mice, WT baicalin-treated mice (+ P < 0.05, ++ P < 0.01). WTH, wild-type hypoxic; A2AR−/−H, A2AR−/− hypoxic; s, saline-treated