Fig. 8
Baicalin increased A2AR expression in the hypoxia-induced PAH mouse model. A2AR protein expression levels in the pulmonary arteries were examined by immunochemical analysis with an anti-A2AR antibody (a; n = 10) (×400; scale bars indicate 50 μm). The mean A2AR density was calculated (b; n = 10). A2AR protein expression levels in lung tissues were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Data are presented as the mean ± SD. #Value significantly greater than the corresponding value in saline-treated normoxic mice (# P < 0.05, ## P < 0.01). *Value significantly greater than the corresponding value in hypoxic mice (*P < 0.05, **P < 0.01). §Value significantly greater than the corresponding value in baicalin-treated mice (§ P < 0.05, §§ P < 0.01). WTH, wildtype hypoxic; s, saline-treated