Fig. 7
From: Lrp, a global regulator, regulates the virulence of Vibrio vulnificus
lrp promoter activity in the absence or presence of Lrp or Lrp*. a Lrp in total cell lysates of the various strains cultured in LB broth for the indicated periods was detected by Western blotting with anti-Lrp antibodies (upper panel). The lower panel is the result of Coomassie blue stain of one of the duplicated gels to show that similar amount of proteins was loaded in each lane for a given strain. b The mRNA levels of lacZ and lrp in bacteria cultured in LB broth for the indicated periods were determined by qRT-PCR. 23S rRNA was used as internal control. The ratio of each mRNA level relative to that of strain WT36 at 2 h is presented. n = 3. The results of no significant difference analyzed by one-way ANOVA along with Tukey’s test are labeled with the same letters, and those of significant differences (P < 0.05) are labeled with different letters. CP212: YJ016ΔlacZ;WT36: YJ016Plrp-lacZ;WT37: YJ016ΔlrpPlrp-lacZ;WT38: YJ016lrp*Plrp-lacZ