Table 1 Bacterial strains and plasmids used in this study
From: Lrp, a global regulator, regulates the virulence of Vibrio vulnificus
Strain/Plasmid | Description | Source/Reference |
|---|---|---|
Escherichia coli | ||
 S17-1λpir | Thi thr leu tonA lacY supE recA::RP4–2 (Km::Tn7,Tc::Mu-1) lysogenized with λpir | [21] |
 KA014 | BL21(pMO08) | This study |
 WT29 | ArcticExpress(pMO08) | This study |
Vibrio vulnificus | ||
 YJ016 | Clinical isolate from blood | [30] |
 NY303–2 | YJ016∆vpl∆vvhA | This study |
 NY303 | Spontaneous attenuated mutant of NY303–2 | This study |
 YH01 | YJ016∆lrp, Cmr | This study |
 YH02 | NY303–2∆lrp, Cmr | This study |
 YH03 | YH01(plrp) | This study |
 YH04 | YH02(plrp) | This study |
 YH05 | NY303(plrp) | This study |
 YH06 | YJ016 containing lrp* cloned from NY303 | This study |
 CH08 | NY303–2 containing lrp* cloned from NY303 | This study |
 CS9133 | Clinical isolate (Biotype 1) | [21] |
 CH09 | CS9133Δlrp, Cmr | This study |
 CECT4999 | Eel pathogen (Biotype 2) | [31] |
 CH10 | CECT4999Δlrp, Cmr | This study |
 KA023 | YH01(pYU01) | This study |
 WT36 | YJ016 integrated with Plrp-lacZ in lacZ | This study |
 WT37 | YH01 integrated with Plrp-lacZ in lacZ | This study |
 WT38 | YH06 integrated with Plrp-lacZ in lacZ | This study |
 HL128 | YJ016∆rtxA1 | [18] |
 CP212 | YJ016∆lacZ | [44] |
Plasmid | ||
 pUC19 | Cloning vector; Apr | [65] |
 pCVD442 | Cloning vector, mob RP4, sacB, and Apr | [66] |
 pIT009 | pJRD215 derivative with the Smr gene replaced by the MCS-containing lacZ gene cloned from pUC19 | [31] |
 plrp | pIT009 inserted with lrp cloned from strain YJ016 | This study |
 pKCW01 | pBBR1MCS4 inserted with mazEF cloned from pR99 at XbaI site | |
 pYU01 | pKCW01 inserted with lrp of strain YJ016, T7 terminator and His6-tag | This study |
 pMO08 | pET30a(+)inserted with lrp cloned from strain YJ016 | This study |
 pMO15 | pCVD442 inserted with Plrp-lacZ | This study |