Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Lrp, a global regulator, regulates the virulence of Vibrio vulnificus

Strain/Plasmid Description Source/Reference
Escherichia coli
 S17-1λpir Thi thr leu tonA lacY supE recA::RP4–2 (Km::Tn7,Tc::Mu-1) lysogenized with λpir [21]
 KA014 BL21(pMO08) This study
 WT29 ArcticExpress(pMO08) This study
Vibrio vulnificus
 YJ016 Clinical isolate from blood [30]
 NY303–2 YJ016∆vplvvhA This study
 NY303 Spontaneous attenuated mutant of NY303–2 This study
 YH01 YJ016∆lrp, Cmr This study
 YH02 NY303–2∆lrp, Cmr This study
 YH03 YH01(plrp) This study
 YH04 YH02(plrp) This study
 YH05 NY303(plrp) This study
 YH06 YJ016 containing lrp* cloned from NY303 This study
 CH08 NY303–2 containing lrp* cloned from NY303 This study
 CS9133 Clinical isolate (Biotype 1) [21]
 CH09 CS9133Δlrp, Cmr This study
 CECT4999 Eel pathogen (Biotype 2) [31]
 CH10 CECT4999Δlrp, Cmr This study
 KA023 YH01(pYU01) This study
 WT36 YJ016 integrated with Plrp-lacZ in lacZ This study
 WT37 YH01 integrated with Plrp-lacZ in lacZ This study
 WT38 YH06 integrated with Plrp-lacZ in lacZ This study
 HL128 YJ016∆rtxA1 [18]
 CP212 YJ016∆lacZ [44]
Plasmid
 pUC19 Cloning vector; Apr [65]
 pCVD442 Cloning vector, mob RP4, sacB, and Apr [66]
 pIT009 pJRD215 derivative with the Smr gene replaced by the MCS-containing lacZ gene cloned from pUC19 [31]
 plrp pIT009 inserted with lrp cloned from strain YJ016 This study
 pKCW01 pBBR1MCS4 inserted with mazEF cloned from pR99 at XbaI site This study; [31, 67]
 pYU01 pKCW01 inserted with lrp of strain YJ016, T7 terminator and His6-tag This study
 pMO08 pET30a(+)inserted with lrp cloned from strain YJ016 This study
 pMO15 pCVD442 inserted with Plrp-lacZ This study
  1. Km kanamycin-resistance gene, Tc tetracycline- resistance gene, Cm r chloramphenicol-resistant, Ap r ampicillin-resistant, Sm r streptomycin-resistance, MCS multiple-cloning site