Fig. 2

Morphological change and phosphorylation levels of phagocytosis-related kinases of macrophages after infection by WT strain or MD mutant. RAW 264.7 cells (a, b) and mPEM (b, d) were coincubated with the WT strain (YJ016) or MD mutant (HL128) at MOI 10 for the indicated periods. a, b The morphological change of infected cells was examined by time-lapse microscopy. Bar = 100 μm. c, d The infected cells were lysed, and the total amount and phosphorylation level at the indicated amino acid residue(s) of each kinase were estimated by immunoblotting with relevant antibodies. Data are representative of three independent experiments