Fig. 5

Biotransformation capacity in the livers. At the time point indicated, mice were sacrificed and the livers were collected for biochemical and histological analysis. Ethoxycoumarin-O-deethylation [ECOD] (a), ethylmorphine-N-demethylation [EMND] (b) and pentoxyresorufin-O-depentylation [PROD] (c) activities in 9000 g supernatants are shown exemplarily. Data are given as mean ± standard deviation (SD), n = 4–6 for each group and time point. Statistical significance was determined by using the non-parametric Kruskal-Wallis test, followed by pairwise Mann-Whitney U tests. Statistical comparisons were made versus the control of each group and are denoted as follows: LPS (asterisk, *), PCI (plus, +), CLP (diamond, #). A p value <0.05 (*,+,#) was considered statistically significant; a p value <0.01 (**,++,##) and a p value <0.001 (***,+++,###) are further specified. d: CYP 3A2 isoforms expressions in liver tissue as determined by immunohistochemistry (red-brown color, counterstaining with hematoxylin). Representative photomicrographs from one of 4–6 different liver tissue samples are shown (original magnification: 400×). 0 h, 4 h, 12 h, 24 h and 72 h were chosen as representative time points to depict the course