Virulence factors, antifungal susceptibility and molecular mechanisms of azole resistance among Candida parapsilosis complex isolates recovered from clinical specimens

Table 1 The sequences of primers and probes used in RT-qPCR

Gene		Primers and probes
ACT1	F	5′- CGAACGTGGTTACGGTTTCT- 3’
	R	5′- TGACCATCTGGCAATTCGTA - 3’
	Probe	TET-TGCAAACCTCATCACAATCA-MGB
CDR1	F	5′- GCTGTTGATCAAAGGGGTGT - 3’
	R	5′- ATCCAAAATCCAGGCAACTG - 3’
	Probe	FAM- CTGATAATGCCGCCAATCTT-MGB
ERG11	F	5′- TGTTGCATTTGGCTGAGAAG - 3’
	R	5′- TCTGAGGGTTTCCTTGATGG - 3’
	Probe	FAM-GGTAAAGGTGGCAACTTGGA-MGB
MDR1	F	5′- TCCCCATTGCTATTGTTGGT - 3’
	R	5′- TGCGCCCATATAATTGAACA - 3’
	Probe	FAM- TTGGTCGGCAACGACATATA-MGB
MRR1	F	5′- CAGCTGCAACAACCACAACT - 3’
	R	5′- TATCATCTAGGCCGCCATTC - 3’
	Probe	FAM- GCAACCACAGCCTATAGGGA-MGB

ISSN: 1423-0127