Table 1 The sequences of primers and probes used in RT-qPCR
Gene | Â | Primers and probes |
|---|---|---|
ACT1 | F | 5′- CGAACGTGGTTACGGTTTCT- 3’ |
R | 5′- TGACCATCTGGCAATTCGTA - 3’ | |
Probe | TET-TGCAAACCTCATCACAATCA-MGB | |
CDR1 | F | 5′- GCTGTTGATCAAAGGGGTGT - 3’ |
R | 5′- ATCCAAAATCCAGGCAACTG - 3’ | |
Probe | FAM- CTGATAATGCCGCCAATCTT-MGB | |
ERG11 | F | 5′- TGTTGCATTTGGCTGAGAAG - 3’ |
R | 5′- TCTGAGGGTTTCCTTGATGG - 3’ | |
Probe | FAM-GGTAAAGGTGGCAACTTGGA-MGB | |
MDR1 | F | 5′- TCCCCATTGCTATTGTTGGT - 3’ |
R | 5′- TGCGCCCATATAATTGAACA - 3’ | |
Probe | FAM- TTGGTCGGCAACGACATATA-MGB | |
MRR1 | F | 5′- CAGCTGCAACAACCACAACT - 3’ |
R | 5′- TATCATCTAGGCCGCCATTC - 3’ | |
Probe | FAM- GCAACCACAGCCTATAGGGA-MGB |