Fig. 2

AA treatment changes the intracellular ROS metabolism at the early stage of differentiation. a Intracellular ROS levels has been examined by flow cytometry in proliferating cells treated with 1 mM H₂O₂ (positive control) for 1 h. b ROS levels were also assessed along the first day of differentiation. Results were compared between untreated and AA-treated differentiating cells. n = ~ 3.10⁴ cells per time point. Values are mean ± SD of three independent experiments. *P ≤ 0.05 compared with untreated differentiating cells at t = 0 h; ^# P ≤ 0.05 between each treatment condition at each time point; ns, non-significant