Fig. 4

The NF-κB signaling pathway is involved in Id-1-mediated invasiveness of NSCLC cells. a and b Representative results (a) and Quantification (b) of migration and invasion of H226 cells, which were stably transfected with sh-Id-1 or negative control with or without TNF-α stimuli measured by Chamber assay. ^* p < 0.05. c Western blotting showing total and phosphorylated IκBα in Id-1-overexpressing A549 cells after treatment without or with JSH-23 (JSH). β-Actin was a loading control. d and e Representative results (d) and Quantification (e) of migration and invasion of A549 cells stably expressing Id-1 with or without JSH-23 measured by Chamber assay. ^* p < 0.05