Fig. 3

OSS-activation of Smad2 is regulated by β1 integrin in ECs plated on FN, and by β1 and β3 integrins in ECs plated on LM. ECs plated on FN (a and b) or LM (c and d) were transfected with control siRNA (siCL) or specific siRNA (30 nM each) of β1 (siβ1) or β3 (siβ3) integrin for 48 h. The cells were kept under static conditions (C) or subjected to OSS (0.5 ± 4 dyn/cm²) for 1 h (a and c) or 24 h (b and d). The phosphorylation of Smad2 and expressions of β1 and β3 integrins were determined by using Western blot analysis. Data are means ± SEM from three independent experiments. ^*P < 0.05 vs. static control cells. ^#P < 0.05 vs. siCL-transfected cells