Fig. 2

ELISPOT data. Mice (C57BL/6, n = 10) were implanted on the left flank with C3 tumours and vaccinated 5 days later with either DPX-R9F, w/o-R9F, DPX (containing no antigen), or untreated. Vaccinations were given subcutaneously on the right flank. Mice (n = 5) were terminated 7 days (a) and 14 days (b) after immunization and IFN-γ ELISPOT performed using splenocytes. Naïve, untreated mice were also included in each experiment. Total lymph node cell counts were performed on the vaccine draining, right inguinal lymph nodes (c) and tumour draining left inguinal lymph nodes (d) at each time point