Fig. 5
Graphs demonstrating volumetric changes in inguinal lymph nodes and tumours over the course of the study comparing DPX-R9F (n = 5) to Squal w/o-R9F (n = 7) and Sap/Lip-R9F (n = 6) and Immune Response by ELISPOT. a Tumour volume time course (mm3). b Normalized % amount SPIO-R9F remaining at the SOI. c % Right lymph node (RLN) volume increase over time (draining vaccine site). d Ratio of RLN volume over LLN volume. Data on graphs is mean ± SE. Significance was calculated using a 2-way ANOVA to compare overall differences across groups and time, and Holm-Sidak corrected t-tests to compare group differences at individual time points, ** = p < 0.05. e Immune response by IFN-γ ELISPOT. HLA-A2 transgenic mice (n = 5) were vaccinated with a mixture of peptides derived from the survivin protein: SurA1.T, SurA2.M, SurA3.K, SurA24, SurB7 as well as a universal T helper epitope A16L. Peptides were formulated in DepoVax, w/o-emulsion with ISA 51, O/W with squalene, or aqueous liposomes. Eight days after immunization mice were euthanized and spleens removed for IFN-γ ELISPOT. Splenocytes (500,000 cells/ well) were stimulated in duplicate with media alone, an irrelevant peptide (HLA-A2 restricted peptide ALMEQQHYV), or SurA2.M peptide. Results shown as average per group +/− SEM. Statistics by one-way ANOVA comparing responses to SurA2.M stimulation, no statistical significance detected