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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Respiratory syncytial virus prolifically infects N2a neuronal cells, leading to TLR4 and nucleolin protein modulations and RSV F protein co-localization with TLR4 and nucleolin

Fig. 6

The potential interactive effects of TLR4 and nucleolin with the RSV F protein were assessed using a co-immunoprecipitation assay. The TLR4 and nucleolin proteins were pulled down with F protein, and their coalition in N2a cells was augmented after RSV infection but not throughout the entire infection period. Crude extract (input) and immunoenriched (IP) fractions were subjected to Western blot analysis. Tagged proteins were detected using an anti-RSV F protein antibody. Anti-mouse IgG served as the negative control

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Journal of Biomedical Science

ISSN: 1423-0127