Fig. 2

CXCL12-induced SMAD3 phosphorylation and SMAD3-lucifease activity in human lung fibroblasts (WI-38). a WI-38 cells were exposed to 10 ng/ml of CXCL12 for 0~ 60 min. Protein levels of phospho-SMAD3 Ser208 and SMAD3 in cell lysates were determined by western blot. Statistics are showed as the mean ± S.E.M. of three separate experiments. * p < 0.05, compared with control without CXCL12 treatment. b Transfection of WI-38 cells with 0.5 μg of SMAD3-Luc and 0.2 μg of pBK-CMV-Lac Z for 24 h. The WI-38 cells were exposed to CXCL12 (1~ 10 ng/ml) for additional 16 h. Luciferase activity assay were described in “Materials and Methods”. Statistics are showed as the mean ± S.E.M. of three separate experiments. * p < 0.05, compared with control without CXCL12 treatment