Fig. 5

Rat model assay. a Rat tibiae growth plate sections from the control group, the Dex-treated group or the Gas5 overexpression lentivirus-infected group were prepared for staining analysis: HE staining (100 ×) for growth plate chondrocytes, IHC staining for FGF1 (400 ×) and active caspase 9 (400 ×), fluorescence in situ hybridisation for miR-21 (200 ×). b The weight, tibiae length, nose-tail length and BALP expression analysis of rats from the control group, the Dex-treated group or the Gas5 overexpression lentivirus-infected group. Data are presented as mean ± s.d., n = 10. *p < 0.05 or **p < 0.01 vs. control group. (c) GAS5 and FGF1 expressions in Dex-treated and untreated chondrocytes