Fig. 5
MPT0B098 inhibits hypoxia-induced F-actin rearrangement and FAK phosphorylation. a Immunofluorescent analysis of F-actin. OEC-M1 cells were treated with various concentrations, indicated as fold of IC50, of MPT0B098 for 18 h under hypoxic conditions, stained with phalloidin to label F-actin (red), counterstained with DAPI (blue), and then observed using an OLYMPUS florescence microscope. b Effect of MPT0B098 on FAK phosphorylation and expression. OEC-M1 cells were treated with various concentrations of MPT0B098 for 18 h.At the end of the drug treatment, cell lysates were prepared and analyzed by SDS-PAGE and Western blot. β-Actin was used as an internal control. c Each bar depicts the mean of the relative intensities of Twist and SNAI2/Slug from three independent experiments (* p < 0.05, compared to hypoxia control)