Fig. 7
MPT0B098 downregulates TGF-β signaling by decreasing the expression of TGF-β mRNA and protein in OEC-M1 cells under hypoxic conditions. a Effect of MPT0B098 on the expression levels of TGF-β1 and TGF-β2 mRNA. Cells were treated with various concentrations of MPT0B098 in hypoxia. After incubation for 36 h, total RNA was extracted, reverse transcribed into cDNA, and subjected to PCR for detection of TGF-β1 and TGF-β2. GAPDH was used as an internal control. Data are represented as mean ± SD in triplicate. **P < 0.01 and ***P < 0.001for comparison between the control and treatment groups using an unpaired two-tailed Student’s t test. b Effect of MPT0B098 on the expression level of TGF-β protein. Cells were treated with various concentrations of MPT0B098 for 36 h in hypoxia. Quantification of TGF-β protein was determined by normalization with GAPDH (* p < 0.05, compared to hypoxia control). c The inhibitory effect of MPT0B098 on TGF-β under normoxic conditions. Cells were treated with MPT0B098 in normoxia at the indicated concentrations for 36 h. Quantification of TGF-β protein was determined by normalization with GAPDH (* p < 0.05, compared to control)