Fig. 2

Subcellular localization of eGFP fusion proteins containing wild-type SP110 or SP110 mutants. a Schematic representation of eGFP fusion proteins containing SP110 deletion mutants. SP100: SP100 domain; SAND: SAND domain; PHD: PHD finger; Bromo: Bromodomain. b–d HEK293T cells were transfected with the indicated constructs, and the cellular distribution of eGFP and eGFP-SP110 fusion proteins (wild-type and mutated forms) was visualized using confocal microscopy at 2 days post-transfection (upper panels). The cells were also subjected to Hoechst staining to identify nuclei (lower panels). Scale bars: 10 μm. The experiments were done at least twice