Fig. 4

Effects of fibroblast growth factor (FGF) receptor 1 (FGFR1) knockdown on phosphorylated extracellular signal-regulated kinase (p-ERK) protein, and β-myosin heavy chain (β-MHC) mRNA expressions in HL-1 cells. (a) Representative immunoblots and average data (n = 6) of FGFR1 from control and FGFR1-knockdown cells (left panel). FGF-2 (25 ng/mL for 30 min) did not increase ERK phosphorylation in FGFR1-knockdown cells without and with calcitriol (10 nM) treatment (right panel). (b) FGF-2 (25 ng/mL for 48 h) did not increase β-MHC mRNA expression in FGFR1-knockdown cells (n = 6). * p < 0.05