Fig. 3

Susceptibility of the double mutants to oxidative and nitrosative stress. a Susceptibility of the mutants to vitamin C (VC) (5 mM for ~ 72 h). Every double mutant was more sensitive than its parent single mutant strains; however, ΔegtA-mshA appeared to be the most sensitive. Data are representative of two experiments. b Susceptibility of the mutants to diamide (DIM) (0.2 mM for ~ 72 h), ΔegtA-mshA is the most sensitive mutant. Data are representative of three independent experiments c Susceptibility of the mutants to sulfaguanidine (Su, 3.8 μg/ml), rifampicin (RIF, 0.0007 μg/ml), streptomycin (Strp, 0.16 μg/ml) and ethambutol (Emb, 0.6 μg/ml). ΔegtA-mshA is more sensitive than its parent strains to RIF and Su, while ΔmshA is sensitive to Strep and ΔegtA is sensitive to EmB. Data are representative of two experiments and two technical replicates for each experiment. d Susceptibility of the mutants to nitric oxide generated by diethylaminetriamine nitric oxide adduct (DETA-NO) (4 mM for 14 days). Even though, the single mutants display a sensitivity trend, ΔegtA-mshA is more sensitive than its parent strains. Data are representative of two experiments and two technical replicates for each experiment. Statistical analyses were performed with Prism using a multiple t-test approach, assuming a uniform distribution with alpha set to 0.05 *(P < 0.05), **(P < 0.01), ***(P < 0.001), ****(P < 0.0001)