Fig. 5

Cell proliferation and viability assays: (a) and (b) Bar graphs showing mean absorbance values obtained from MTT assay in U-87MG and LN-18 Cell lines respectively after treatment with 10 μM reparixin-l-lysine at different time intervals-24, 48 and 72 h. c Ki-67 labelling done by immunocytochemistry in U-87MG and LN-18 cells. The bar graph is showing percentage positivity of Ki-67 staining in both the cell lines. Student’s t-test was performed to analyze the statistical difference in percentage positivity of Ki-67 labelling between control and reparixin-l-lysine (10 μM) treated cells in both the cell lines. A p-value of less than 0.05 was considered statistically significant. d and e Clonogenic assay in U-87MG and LN-18 cell lines after treatment with Reparixin-l-lysine. Error bar indicates standard error calculated from three independent experiments in triplicate