Fig. 4
From: L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts
l-CaD facilitates the cell-cell fusion in RANKL-induced OCs. (a) RANKL-induced TRAP-positive OC images with TRAP staining (red, top), and DAPI nuclei staining (white, middle), and merged color micrographs showing TRAP staining in red, and nuclei in blue in differentiated cells with si Ctr or si-l-CaD treatment. Fusion index was determined by measuring the portion of clustered nuclei to total nuclei in cells by using Image-Pro Plus computerized software. Quantitative analyses for RANKL-induced cell fusion index in OCs treated with si Ctr or si-l-CaD were shown at the bottom. b RANKL-induced TRAP-positive OC images with TRAP staining (red, top), and DAPI nuclei staining (white, middle), and merged color micrographs showing TRAP staining in red, and nuclei in blue in OCs with EGFP or EGFP-l-CaD transfection. Quantitative analyses for RANKL-induced cell fusion index in OCs transfected with EGFP or EGFP-l-CaD expressions were shown at the bottom. Calibration scale in (a) and (b) as indicated. In (a) and (b), the values are the mean ± SEM (n = 6), with *, # indicating a significant difference compared to the si Ctr-treated or EGFP-transfected cells, respectively