Fig. 4

Promoter hypermethylation silences expression of ZNF667-AS1 or ZNF667. a. Promoter activity of different fragments of ZNF667-AS1 (− 636/+ 52, − 173/+ 52, and + 53/+ 545). The luciferase activity of pGL3- region 2 vector was set to 1. In vitro methylation of region 2 led to a significant decrease in luciferase activity of ZNF667-AS1. The schematic structures of the promoter fragments were listed below. b. Promoter activity of different fragments of ZNF667 (− 543/− 275, and − 270/21). The luciferase activity of pGL3- region 2 vector was set to 1. In vitro methylation of region 2 led to a significant decrease in luciferase activity of ZNF667. The schematic structures of the promoter fragments were listed below. *P < 0.05, **P < 0.01