Group | N | Methylation frequency | |||||
---|---|---|---|---|---|---|---|
Region 1 | Region 2 | Region 3 | |||||
 |  | n (%) | P | n (%) | P | n (%) | P |
Age | Â | Â | Â | Â | Â | Â | Â |
 <60 | 13 | 8 (61.5) | 1.0 | 8 (61.5) | 0.473 | 10 (76.9) | 0.2673 |
  ≥ 60 | 19 | 12 (63.2) |  | 8 (42.1) |  | 10 (52.6) |  |
Smoking | Â | Â | Â | Â | Â | Â | Â |
 Positive | 27 | 18 (66.7) | 0.338 | 14 (51.9) | 1.0 | 18 (66.7) | 0.338 |
 Negative | 5 | 2 (40.0) |  | 2 (0.4) |  | 2 (40.0) |  |
Drinking | Â | Â | Â | Â | Â | Â | Â |
 Positive | 19 | 11 (57.9) | 0.713 | 9 (47.4) | 1.0 | 14 (73.7) | 0.150 |
 Negative | 13 | 9 (69.2 |  | 7 (53.8) |  | 6 (46.2) |  |
Pathological differentiation | Â | Â | Â | Â | Â | Â | Â |
 Well | 15 | 9 (60.0) | 1.0 | 4 (26.7) | 0.032 | 7 (46.7) | 0.144 |
 Moderate/poor | 17 | 11 (64.7) |  | 12 (70.6) |  | 13 (76.5) |  |
TNM stage | Â | Â | Â | Â | Â | Â | Â |
 I + II | 14 | 11 (78.6) | 0.147 | 8 (57.1) | 0.722 | 10 (71.4) | 0.471 |
 III + IV | 18 | 9 (50.0) |  | 8 (44.4) |  | 10 (55.6) |  |
LN metastasis | Â | Â | Â | Â | Â | Â | Â |
 Positive | 12 | 7 (58.3) | 0.734 | 7 (58.3) | 0.716 | 7 (58.3) | 0.724 |
 Negative | 20 | 13 (65.0) |  | 9 (45.0) |  | 13 (65.0) |  |
Tissues | Â | Â | Â | Â | Â | Â | Â |
 Tumor | 32 | 20 (62.5) | < 0.001 | 16 (50.0) | < 0.001 | 20 (62.5) | < 0.001 |
 Normal | 32 | 3 (9.3) |  | 1 (3.1) |  | 4 (12.5) |  |