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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Loss of NFBD1/MDC1 disrupts homologous recombination repair and sensitizes nasopharyngeal carcinoma cells to PARP inhibitors

Fig. 1

Knockdown of endogenous NFBD1 using lentivirus-mediated shRNA. The lentiviral expressing shNFBD1 and shControl were transfected into CNE1, CNE2 and HNE1 cells. The stable NFBD1-deficiency cells were obtained under puromycin (1 μg/ml). (a) Effects of constructed lentiviral on the expression of NFBD1 mRNA were determined by qRT-PCR. The relative expression level of NFBD1 mRNA was significantly downregulated in NFBD1-shRNA group, P < 0.01 compared with shControl group. Effects of constructed lentiviral on the expression of NFBD1 protein were determined by western blotting (b) and immunofluorescence (c). **P < 0.01

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