Fig. 4

Microvasculature profile of the mouse hippocampus treated with Stx2 or Stx2 + LPS. Control (a-b), LPS (c-d), Stx2 (e-f) and Stx2 + LPS (g-h) after 2 days of treatments. Representative lectin histofluorescence micrographs (a, c, e, g). Merge micrographs between lectin and Hoechst histofluorescences (b, d, f, h, l). Number of glycocalyx positive particles at 2, 4, 7 and 20 days (j). Area occupied by glycocalyx in microvessels from different treatments at 2, 4, 7 and 20 days (k). The area analyzed in this study was Rad from the CA1 hippocampal area (drawn rectangle) (l). Negative control by omitting a primary antibody (i). Different letters (a, b, c, d) above the columns indicate a significant difference between the 4 different i.v.-treated groups (j-k). The arrows indicate the areas where the lectin-binding glycocalix was not express by endothelial cells (g). The scale bar in Fig. I applies to all micrographs. Data were analyzed by One-way ANOVA, Bonferroni’s post hoc test, p < 0.05, n = 4