Fig. 1

Plzf temporarily expressed in Pax6⁺ cells at early stages of brain development. a, Western blot analysis of Plzf expression in whole brain lysates of mouse embryo at different stages. b-d, 20 μm frozen sections were prepared from the E9.5 (b), E10.5 (c), and E11.5 (d) embryos and immunohistochemistry for Plzf was performed with DAB (brown) followed by Hematoxylin-eosin (HE) counterstaining. The diagrams of mouse embryo were obtained from e-Mouse Atlas Project (http://www.emouseatlas.org/emap/ema/home.php) and the green circles indicate the approximate plane of the cross section. The higher magnification images of the black boxes were shown in the right panels. Scale bar, 20 μm. e, The expressions of Pax6 (red) and Plzf (brown) were examined by immunostaining in the same brain sections. The higher magnification images of the white boxes were shown in the right panels. E, embryonic day; nt, neural tube; tv, third ventricle; tel., telencephalon; di, diencephalon. Scale bar, 10 μm