Fig. 7

Pharmacological or genetic suppression of HDAC6 phenocopied stroke induced retardation in dendritic maturation. (a) Schematic illustration of the procedure for pharmacological suppression of HDAC6 on the dendritic maturation in vivo. Tubastatin A or ACY-738 were applied intraperitoneally every two days. (b) Representative images of the retroviral labeled newly generated neurons at 14 dpi. The scale bar is 25 μm. (c and d) Quantification of total dendritic length and the number of dendritic branches of individual newborn neurons at 14 dpi from different groups. Data are presented as mean ± SEM. n = 20 to 40 cells from four mice, data are analyzed by one-way ANOVA with Bonferroni’s post hoc analysis. * p < 0.05 compared to vehicle injected control. (e) Schematic illustration of the procedure for genetic silencing of HDAC6 on the dendritic maturation in vivo. pU6: U6 promoter, shHDAC6: shRNA targets HDAC6. (f) Representative images of the newly generated neurons at 14 dpi. The scale bar is 25 μm. (g and h) Quantification of total dendritic length and the number of dendritic branches of individual newborn neurons at 14 dpi from different groups. Data are presented as mean ± SEM. n = 20 to 40 cells from four mice, data are analyzed by one-way ANOVA with Bonferroni’s post hoc analysis. * p < 0.05 compared to shDsRed control