Fig. 1From: Fibronectin inhibitor pUR4 attenuates tumor necrosis factor α–induced endothelial hyperpermeability by modulating β1 integrin activationSpinal nerve ligation induces excessive FN deposition around microvessels in the spinal cord. The L5 spinal nerve distal to the dorsal root ganglion of rats was tightly ligated. a Representative photomicrograph depicting immunofluorescence analysis for FN on the operated and contralateral sides of the L5 segment of the spinal cord 7 days after surgery. b Regional magnification of the boxed area in (a); FN+ microvessel-like profiles are indicated by arrows. c Percentages of the FN+ area in the total area on the operated and contralateral sides of the L5 segment of the spinal cord were analyzed (n = 3). Data are presented as means ± standard errors of means. **P < 0.01, Student’s t test. d and e Representative images at low (d) and high (e) magnification showing immunocytochemistry of FN in the L5 dorsal part of the spinal cord. Arrows indicate FN+-microvessel-like profiles on the operated side of the spinal cord. f Immunoblotting for FN expression in the pooled L5 dorsal spinal cord on the operated and contralateral sides in five male Sprague Dawley rats. Equal protein loading was confirmed with α-tubulin. Quantification of immunoblotting of FN normalized to α-tubulin in tissues is shown. g–i Confocal microscopic images of FN+-microvessel-like profiles (red; g) and collagen IV+ capillaries (green; h) in the L5 dorsal part of the spinal cord; merged images (i) showing the colocalization of FN and collagen IV (yellow) in the capillaries are indicated with arrowheadsBack to article page