Fig. 2

Representative western blot analysis and quantification of temporal fold-changes relative to sham-controls of Pnn (a) or cleaved Caspase-3 (b) in the ipsilateral cortex and striatum of sham-controls or rats after 90 min of MCAO or 6 h and 24 h after MCAO/R. Values are mean ± SEM of individual samples obtained from 3 to 4 animals per experimental group. *P < 0.05 versus sham-control (S) group in the post hoc Scheffé multiple-range analysis. c Illustrative example of co-localization (white arrow) of Pnn (green fluorescence) and GFAP (red fluorescence) immunoreactivity in reactive astrocytes at the peri-infarct area of ipsilateral cerebral cortex in rats after MCAO/R. Scale bar: 20 μm