Fig. 5

Representative western blot analysis and quantification of temporal fold-changes relative to nonspecific (si-Ctrl) controls of Pnn (a), phospho-ERK/ERK (b) or Bcl-2 (c) expression in rat primary cultured astrocytes under normoxic conditions, after 24 h of exposure to oxygen-glucose deprivation (OGD), or after 24 h of re-oxygenation (OGD/R); and additionally received treatment with Pnn specific siRNA (si-Pnn). Values are mean ± SEM of 5–6 independent experiments. *P < 0.05 versus normoxic control (Ctrl) group, ⁺P < 0.05 versus OGD group, and ^#P < 0.05 versus nonspecific siRNA group (si-Ctrl) in the post hoc Scheffé multiple-range analysis