Fig. 1

TREM-1 deficiency exacerbates DSS-induced colitis in mice. WT and TREM-1 KO mice (n = 27/group) were supplied with normal drinking water or drinking water containing 3% DSS for 5 days, followed by normal drinking water for 4 days. a Weight change, b images of colon length and cecum size (one square = 1 cm), c cecum weight, d quantitation of colon length, e survival rate, and f UCDAI score were all determined on day 9. Data in a, c, d, and f are the mean ± SEM. P values of differences were determined by Mann-Whitney nonparametric test. *, p < 0.05; **, p < 0.01; ***, p < 0.001 or ****, p < 0.0001. Survival rate was compared using the log-rank test, *, p < 0.05. g Representative images of histopathology of sections of colonic tissues that were prepared from water-treated WT and TREM-1 KO mice (n = 17/group) and DSS-treated WT and TREM-1 KO mice (n = 27/group) and stained with HE or AB-PAS. Data are representative of 7 independent experiments. h Histopathological scores of the DSS-treated mice in (g). Data are the mean ± SEM. i Epithelial barrier permeability as determined by measuring FITC-dextran levels in the serum of the WT and TREM-1 KO mice in (g) on day 9 after DSS initiation. Data are pooled from two independent experiments involving 6 mice per group and are the mean ± SEM