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Table 1 Mutation frequency of influenza A/H1N1 viruses based on conventional sequencing analysis

From: Naturally occurring mutations in PB1 affect influenza A virus replication fidelity, virulence, and adaptability

  1. Wild-type PR8 and PR8PB1(S216G) viruses were passaged twice in MDCK cells. The HA cDNA was amplified from viral supernatant, and cloned into pJET1.2/blunt as described in the Methods. The HA mutation frequency was determined by clonal sequencing of 94 to 105 bacterial clones for each experiment were picked and repeated independently three times. *by Student’s two-tailed unpaired t-test; NS Not significant