Fig. 1

Expression of hsa-miR-1975 in A549 cells after influenza virus infection. a Outline of the identification of small RNAs involved in influenza A virus infection. A549 cells were infected with influenza virus (A/WSN/33) at an MOI of 5 or uninfected. At 25 h p.i.(hours post infection), small RNAs ranging from 18 to 40 nucleotides were collected and processed for RNA sequencing. The interesting small RNA was identified and selected for further validation. b A549 cells were infected with WSN (MOI = 5) and harvested at different time points for RT-qPCR analysis. The RNA levels of hsa-miR-1975 were normalized by U24 RNA. Values represent the mean ± SD of three independent experiments. c A549 cells were infected with different strains of influenza A virus (MOI = 0.01) for 40 h and then harvested for RT-qPCR analysis. The RNA levels of hsa-miR-1975 were normalized by U24 RNA. Values represent the mean ± SD of four independent experiments. Statistical comparisons between groups by one-way ANOVA with Bonferroni correction for multiple comparisons (b and c). Statistically significant differences are indicated as follows: *P < 0.05, **P < 0.01, and ***P < 0.001