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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Glucosamine impedes transforming growth factor β1-mediated corneal fibroblast differentiation by targeting Krüppel-like factor 4

Fig. 5

Effects of GlcN-induced KLF4 mRNA and protein expression on corneal fibrosis in HCFs. (a) Real-time PCR analysis showing the expression levels KLF4, α-SMA, fibronectin, and COL1A1 mRNA in HCFs transfected with siControl or siKLF4. GAPDH served as a loading control. GlcN (5 mM) and/or TGF-β1 (10 ng/ml) were applied for 24 h where indicated (+). Bars depict the mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. (b) Immunoblot analysis for detection of KLF4, α-SMA, fibronectin, and collagen I in HCFs transfected with siControl or siKLF4. GlcN and/or TGF-β1 were applied as indicated. ACTN served as a loading control

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Journal of Biomedical Science

ISSN: 1423-0127